The screen high-throughput liquid handling system separates individual nucleic acid templates into separate reaction chambers for single-molecule quantification through PCR amplification and fluorescence signal detection.Digital PCR technology does not rely on Ct values and standard calibration curves, and has extremely high sensitivity, precision and excellent repeatability.
automation
The screen high-throughput liquid handling system separates individual nucleic acid templates into separate reaction chambers for single-molecule quantification through PCR amplification and fluorescence signal detection.Digital PCR technology does not rely on Ct values and standard calibration curves, and has extremely high sensitivity, precision and excellent repeatability.
genetic research
We detected cfDNA somatic mutations in combination with protein markers to efficiently identify early-stage HCC from asymptomatic HBsAg-seropositive individuals in a community population.
AFP and ultrasound
We designed an assay to analyze multiple types of genetic variation in parallel so that 2 mL of plasma is sufficient to achieve high sensitivity in detecting cfDNA mutations.The assay successfully identified 4 early-stage HCC cases (<3 cm) from 331 HBsAg(+) individuals based on negative serum AFP and ultrasonography.
hepatocellular carcinoma
The positive predictive value was 17%, which was much higher than previous studies.This article shows that the combination of cfDNA and serum protein markers holds great promise for the early detection of HCC in community-based populations.
